[1]王 建,胡立珍,張國翠,等.PNB/TCH生長試驗和DNA微陣列基因芯片鑒定非結核分枝桿菌的價值比較[J].醫學信息,2019,(20):170-171.[doi:10.3969/j.issn.1006-1959.2019.20.054]
 WANG Jian,HU Li-zhen,ZHANG Guo-cui,et al.Comparison of PNB/TCH Growth and DNA Microarray Gene Chips for Identification of Nontuberculous Mycobacteria[J].Medical Information,2019,(20):170-171.[doi:10.3969/j.issn.1006-1959.2019.20.054]
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PNB/TCH生長試驗和DNA微陣列基因芯片鑒定非結核分枝桿菌的價值比較()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2019年20期
頁碼:
170-171
欄目:
診療技術
出版日期:
2019-10-15

文章信息/Info

Title:
Comparison of PNB/TCH Growth and DNA Microarray Gene Chips for Identification of Nontuberculous Mycobacteria
文章編號:
1006-1959(2019)20-0170-02
作者:
王 建1胡立珍1張國翠1趙 輝2陳子芳1
(山東省濱州市中心醫院/濱州市結核病防治院檢驗科1,呼吸內科2,山東 濱州 251700)
Author(s):
WANG Jian1HU Li-zhen1ZHANG Guo-cui1ZHAO Hui2CHEN Zi-fang1
(Department of Clinical Laboratory1,Department of Respiratory Medicine2,Binzhou Central Hospital/Binzhou City Tuberculosis Hospital,Binzhou 251700,Shandong,China)
關鍵詞:
非結核分枝桿菌PNB/TCH基因芯片菌種鑒定
Keywords:
Non-tuberculous mycobacteriaPNB/TCHGene chipStrain identification
分類號:
R440
DOI:
10.3969/j.issn.1006-1959.2019.20.054
文獻標志碼:
A
摘要:
目的 比較對硝基苯甲酸/噻吩-2-羧酸肼生長(PNB/TCH)和DNA微陣列基因芯片鑒定非結核分枝桿菌(NTM)臨床分離株菌種的價值。方法 收集2013年1月~2018年6月我院住院患者的痰分枝桿菌培陽分離菌2175株,分別采用PNB/TCH生長試驗和DNA微陣列基因芯片鑒別NTM,比較兩種方法的鑒定結果。結果 PNB/TCH生長試驗初步鑒定NTM 93株,占4.28%,DNA微陣列基因芯片鑒定NTM 79株,占3.63%,PNB/TCH生長試驗初步鑒定的93株NTM中,有14例為假NTM(15.05%);PNB/TCH生長試驗鑒定NTM的陽性檢出率低于DNA微陣列基因芯片;79株NTM中,占比前3位的依次為胞內分枝桿菌株(37.97%)、堪薩斯分枝桿菌(21.52%)、鳥分枝桿菌(16.46%)。結論 傳統對硝基苯甲酸/噻吩-2-羧酸肼生長試驗鑒定NTM特異性較低,采用DNA微陣列基因芯片技術鑒定NTM準確,臨床參考價值較大。
Abstract:
Objective To compare the growth of non-tuberculous mycobacteria (NTM) clinical isolates with p-nitrobenzoic acid/thiophene-2-carboxylate growth (PNB/TCH) and DNA microarray microarray.Methods A total of 2175 strains of Mycobacterium phlei isolates in our hospital from January 2013 to June 2018 were collected. PNB/TCH growth test and DNA microarray gene chip were used to identify NTM. Identification of the method.Results PNB/TCH growth assay initially identified NTM 93 strain, accounting for 4.28%, DNA microarray gene chip identified NTM 79 strain, accounting for 3.63%, and of the 93 NTMs initially identified by PNB/TCH growth test, 14 were pseudo NTM ( 15.05%); PNB/TCH growth test identified that the positive detection rate of NTM was lower than that of DNA microarray gene chip; among the 79 strains of NTM, the top 3 were followed by intracellular branching strain (37.97%), Kansas Mycobacterium (21.52%), Mycobacterium avium (16.46%). Conclusion The traditional p-nitrobenzoic acid/thiophene-2-carboxylic acid oxime growth test has low specificity of NTM. The DNA microarray gene chip technology is used to identify NTM accurately, and the clinical reference value is large.

參考文獻/References:

[1]全國第五次結核病抽樣調查技術指導組,全國第五次結核病抽樣調查辦公室.2010年全國第五次結核病流行病學抽樣調查報告[J].中國防癆雜志,2012,34(8):485-508. [2]趙雁林,逄宇.結核病實驗室檢驗規程[M].北京:人民衛生出版社,2015. [3]劉海燦,黃明翔,蔣毅,等.福建省肺非結核分枝桿菌臨床分離株的菌種鑒定[J].中國人獸共患病學報,2017,33(5):389-397. [4]柳正衛,吳蓓蓓,鄭琳,等.微陣列芯片技術應用于分枝桿菌菌種鑒定的研究[J].浙江預防醫學,2013,25(8):1-4. [5]余旭泉,徐禮鋒,祝進,等.99株分枝桿菌菌種鑒定和耐藥檢測結果分析[J].中國衛生檢驗雜志,2015,25(20):3580-3582.

更新日期/Last Update: 2019-10-15
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