[1]張小敏,王 潔,王淑靜,等.DLPME-HPLC法同時測定人血清中維生素A和維生素E含量[J].醫學信息,2019,(20):161-163.[doi:10.3969/j.issn.1006-1959.2019.20.051]
 ZHANG Xiao-min,WANG Jie,WANG Shu-jing,et al.Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC[J].Medical Information,2019,(20):161-163.[doi:10.3969/j.issn.1006-1959.2019.20.051]
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DLPME-HPLC法同時測定人血清中維生素A和維生素E含量()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2019年20期
頁碼:
161-163
欄目:
診療技術
出版日期:
2019-10-15

文章信息/Info

Title:
Simultaneous Determination of Vitamin A and Vitamin E in Human Serum by DLPME-HPLC
文章編號:
1006-1959(2019)20-0161-03
作者:
張小敏王 潔王淑靜朱佳佳
(寧夏醫科大學醫學科學技術研究中心,寧夏 銀川 750004)
Author(s):
ZHANG Xiao-minWANG JieWANG Shu-jingZHU Jia-jia
(Ningxia Medical University Medical Science and Technology Research Center,Yinchuan 750004,Ningxia,China)
關鍵詞:
分散液相微萃取高效液相色譜人血清維生素A維生素E
Keywords:
Dispersive liquid phase microextractionHigh performance liquid chromatographyHuman serumVitamin AVitamin E
分類號:
R151.2
DOI:
10.3969/j.issn.1006-1959.2019.20.051
文獻標志碼:
A
摘要:
目的 建立分散液相微萃取(DLPME)-高效液相色譜(HPLC)同時測定人血清中維生素A、E含量的方法。方法 2018年5月隨機抽取人血液樣本50例,經DLPME技術處理后,采用HPLC對人血清中維生素A、E含量進行檢測,使用標準曲線法進行定量分析。結果 維生素A標準曲線方程為y=13225x+410.03,相關系數為0.9998,檢出限為0.042 μg/ml;維生素E標準曲線方程為y=1383.9x-458.27,相關系數為0.9997,檢出限為0.096 μg/ml。維生素A和維生素E的加標回收率分別為90.12%~103.70%和81.09%~92.21%,含量范圍分別為(0.62±0.04)μg/ml和(8.41±0.43)μg/ml。結論 DLPME-HPLC法操作簡便,節約試劑,對樣本富集效率高,通過該方法的研究,對微量血清中維生素A和維生素E的測定具很好的的應用價值。
Abstract:
Objective To establish a method for simultaneous determination of vitamin A and E in human serum by dispersive liquid phase microextraction (DLPME)-high performance liquid chromatography (HPLC). Methods In May 2018, 50 human blood samples were randomly selected. After treatment with DLPME technique, the contents of vitamin A and E in human serum were detected by HPLC and quantified by standard curve method.Results The standard curve of vitamin A was y=13225x+410.03, the correlation coefficient was 0.9998, the detection limit was 0.042 μg/ml; the standard curve of vitamin E was y=1383.9x-458.27, the correlation coefficient was 0.9997, and the detection limit was 0.096 μg/ml. The recoveries of vitamin A and vitamin E were 90.12%~103.70% and 81.09%~92.21%, respectively, and the contents ranged from (0.62±0.04)μg/ml and (8.41±0.43)μg/ml.Conclusion DLPME-HPLC method is easy to operate, saves reagents, and has high efficiency of sample enrichment. The research of this method has a good application value for the determination of vitamin A and vitamin E in trace serum.

參考文獻/References:

[1]石慶平,王鐵華,張浩,等.反相高效液相色譜法檢查復方維生素注射液中的有關物質[J].海軍醫學雜志,2014,5(2):143-147. [2]劉信嘉,林森煜,李秀英,等.正相高效液相色譜法同時測定奶粉中維生素A醋酸酯、維生素A棕櫚酸酯、維生素E醋酸酯及α-生育酚[J].廣東化工,2015,42(5):126-127. [3]賈妍,戰思恩,翟燕紅,等.液相色譜串聯質譜法測定血清維生素A、E含量[J].標記免疫分析與臨床,2018,25(4):574-579. [4]范菲,楊會鴿,周娜,等.HPLC法同時測定多維元素片中維生素A和維生素E的含量[J].西北藥學雜志,2016,31(6):590-592. [5]張航.離子液體液相微萃取有機紫外線吸收劑的研究[D].云南師范大學,2016.

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更新日期/Last Update: 2019-10-15
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