[1]修朋程,李 騰,李雅林,等.泰山山慈菇對小鼠肝癌的治療作用及相關免疫機制研究[J].醫學信息,2018,(09):8-11.[doi:10.3969/j.issn.1006-1959.2018.09.003]
 XIU Peng-cheng,LI Teng,LI Ya-lin,et al.Study on the Therapeutic Effect and Mechanism of Taishan Mountain Mushroom on Liver Cancer in Mice[J].Medical Information,2018,(09):8-11.[doi:10.3969/j.issn.1006-1959.2018.09.003]
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泰山山慈菇對小鼠肝癌的治療作用及相關免疫機制研究()
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醫學信息[ISSN:1006-1959/CN:61-1278/R]

卷:
期數:
2018年09期
頁碼:
8-11
欄目:
終末期肝病的診斷與治療專題
出版日期:
2018-05-01

文章信息/Info

Title:
Study on the Therapeutic Effect and Mechanism of Taishan Mountain Mushroom on Liver Cancer in Mice
文章編號:
1006-1959(2018)09-0008-04
作者:
修朋程李 騰李雅林劉紀豪李 棟劉有旺
泰山醫學院,山東 泰安 271000
Author(s):
XIU Peng-chengLI TengLI Ya-linLIU Ji-haoLI DongLIU You-wang
Taishan Medical College,Tai'an 271000,Shandong,China
關鍵詞:
泰山山慈菇肝癌腫瘤壞死因子-α白細胞介素-2γ-干擾素
Keywords:
Key words:Taishan mountain mushroomLiver cancerTumor necrosis factor -αInterleukin-2γ-interferon
分類號:
R392.3
DOI:
10.3969/j.issn.1006-1959.2018.09.003
文獻標志碼:
A
摘要:
目的 探討泰山山慈菇對小鼠肝癌的免疫治療作用。方法 本課題首先制備泰山山慈菇提取液,體外用山慈菇提取液對H22肝癌細胞進行干預,分為空白組、山慈菇提取液低、中、高劑量組和5-氟尿嘧啶陽性對照組,干預12 h后,Annexin V和7-AAD染色,流式細胞術檢測細胞凋亡。體內制備小鼠H22實體瘤肝癌模型,將50只小鼠分為山慈菇低、中、高劑量組、5-Fu陽性對照組、模型對照組,每組10只。山慈菇各劑量組均進行灌胃治療,模型組灌胃等體積生理鹽水,5-Fu陽性對照組進行等體積腹腔注射。連續 10 d,停藥后次日摘取小鼠眼球取血后頸椎脫臼處死小鼠,檢測小鼠腫瘤大小分析對腫瘤的抑制作用。酶聯免疫吸附試驗(ELISA)檢測血清中腫瘤壞死因子-α(TNF-α)、白細胞介素-2(IL-2)和γ-干擾素(IFN-γ)的含量。結果 泰山山慈菇提取液體外促進H22腫瘤細胞凋亡且具有劑量依賴性。體內抑制H22腫瘤的生長,增加血清中TNF-α、IL-2和IFN-γ的含量且呈現劑量依賴性。結論 泰山山慈菇通過增加抗腫瘤細胞因子產生發揮抗腫瘤作用。
Abstract:
Abstract:Objective To investigate the immunotherapy effect of Taishan mountain mushroom on liver cancer in mice.Methods We first prepared the Taishan mountain mushroom extract.In vitro,the H22 hepatoma cells were treated with the extract of mushroom extract in vitro.It was divided into blank group,low,middle and high dose group and 5- fluorouracil positive control group.After 12 h intervention,Annexin V and 7-AAD were stained,and cell apoptosis was detected by flow cytometry.The mice model of H22 solid tumor was prepared in vivo,and 50 mice were divided into low,medium and high dose group,5-Fu positive control group and model control group,with 10 rats in each group.The rats in each dose group were treated by intragastric perfusion,and the model group was given iso-volume intraperitoneal injection of normal saline and 5-Fu positive control group.For 10 d,the mice were killed the next day after extirpation of eyeball and blood,and the inhibitory effect of tumor size analysis on the tumor was detected.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of TNF-α,IL-2 and IFN-γ in serum.Results The extract of Taishan mountain mushroom promoted the apoptosis of H22 tumor cells in a dose-dependent manner.In vivo,the growth of H22 tumor was inhibited and the levels of TNF-α,IL-2 and IFN-γ in serum increased.Conclusion Taishan mountain mushroom plays an anti-tumor role by increasing the production of anti-tumor cytokines.

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更新日期/Last Update: 2018-05-01
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